LAMP Detection of Virus-Derived DNA of Zika Virus in Vector Mosquito

Detection of infectious viruses in mosquitoes is one the prerequisite measures to monitor prevalence vector-borne viral diseases. Determining which are currently infected with arboviruses such as Zika, dengue, and chikungunya virus not yet practical endemic areas due multiple causes including difficulty dealing virus’ unstable RNA. In this study, instead handling RNA, virus-derived DNA (vDNA) was introduced a target template for nucleic acid amplification. combination loop-mediated isothermal amplification (LAMP), we examined LAMP-based vDNA detection assay (vDNA-LAMP) targeting Zika (ZIKV). The vDNA-LAMP reaction amplifying part NS3 region ZIKV successfully detected its from crude purified artificially cultured cells Aedes mosquitoes. This rapid, simple, versatile method may provide promising field-surveillance arbovirus circulation via vector